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Preparation and evaluation of magnetic molecularly imprinted polymers for the specific enrichment of phloridzin

Gao, Die, Wang, Dan-Dan, Fu, Qi-Feng, Wang, Lu-Jun, Zhang, Kai-Lian, Yang, Feng-Qing, Xia, Zhi-Ning
Talanta 2018 v.178 pp. 299-307
Fourier transform infrared spectroscopy, Malus doumeri, X-ray diffraction, adsorbents, adsorption, baicalin, ethylene glycol, iron oxides, leaves, oral administration, phloridzin, polymers, rats, scanning electron microscopy, transmission electron microscopy
In present study, magnetic molecularly imprinted polymers (MMIPs) were successfully prepared for specific recognition and selective enrichment of phloridzin from the leaves of Malus doumeri (Bois) A. Chev and rats' plasma. The magnetic Fe3O4 were prepared by the solvothermal reaction method and followed by the modification of TEOS and functionalization with APTES. Using functionalized Fe3O4 particles as the magnetic cores, phloridzin as template, ethylene glycol dimethacrylate (EGDMA) as cross-linker and 2,2-azobisisobutyonnitrile (AIBN) as initiator, the MMIPs were prepared through APTES to associate the template on the surface of the magnetic substrate. The structural features and morphological characterizations of MMIPs were performed by FT-IR, SEM, TEM, XRD, TGA and VSM. The adsorption experiments revealed that the MMIPs presented high selective recognition property to phloridzin. The selectivity experiment indicated that the adsorption capacity and selectivity of polymers to phloridzin was higher than that of baicalin and 2,3,5,4′-ttrahydroxy stilbene-2-O-β-D-glucoside. Furthermore, the MMIPs were employed as adsorbents for extraction and enrichment of phloridzin from the leaves of M. doumeri and rats' plasma. The recoveries of phloridzin in the leaves of M. doumeri ranged from 81.45% to 90.27%. The maximum concentration (Cmax) of phloridzin in rats' plasma was detected as 12.19 ± 0.84μg/mL at about 15min after oral administration of phloridzin (200mg/kg). These results demonstrate that the prepared MMIPs are suitable for the selective adsorption of phloridzin from complex samples such as natural medical plants and biological samples.