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Characterization, antioxidant and antiglycation properties of polysaccharides extracted from the medicinal halophyte Carpobrotus edulis L
- Hafsa, Jawhar, Hammi, Khaoula Mkadmini, Cerf, Didier Le, Limem, Khalifa, Majdoub, Hatem, Charfeddine, Bassem
- International journal of biological macromolecules 2017
- 2,2-diphenyl-1-picrylhydrazyl, Carpobrotus edulis, Fourier transform infrared spectroscopy, advanced glycation end products, antioxidant activity, antioxidants, arabinose, esterification, fluorescence, galacturonic acid, halophytes, lipid peroxidation, mannose, models, molecular weight, nuclear magnetic resonance spectroscopy, polysaccharides, rhamnose, temperature, ultrasonics, xylose
- In this study, Box-Behnken design was used to optimize the ultrasonic extraction of Carpobrotus edulis polysaccharides (CEP), and the effect of time, extraction temperature and water to material ratio was evaluated. Optimum conditions were 1.77h, 78.0°C and 33.04mL/g to improved CEP yield (7.84%), which is in good agreement with the predicted yield 7.77%. Then, the physico-chemical, antioxidant and antiglycation properties of optimized CEP were studied, and the total sugar and galacturonic acid content were 89.7 and 63.2%, respectively. The composition of neutral monosaccharide was arabinose, xylose, rhamnose and mannose in the molar percentage of 71.84, 14.80, 8.57, and 4.79%, respectively. In addition, (1H, and 13C) NMR and FTIR analyses confirmed the presence of uronic acids in the free and methyl ester forms with a degree of esterification of 31.27%. Therefore, this finding showed that CEP is a low methoxyl pectic polysaccharide, with an average molecular weight about 65,000g/mol. Finally, the results indicated that CEP presents strong antioxidant activities in vitro (DPPH, chelating ability and reducing power), and significantly inhibits lipid peroxidation and the formation of fluorescent advanced glycation end products in glucose-BSA system model.