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Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii

Author:
Biscaia, S.M.P., Carbonero, E.R., Bellan, D.L., Borges, B.S., Costa, C.R., Rossi, G.R., Gonçalves, J.P., Melo, C.M., Lívero, F.A.R., Ruthes, A.C., Zotz, R., Silva, E.V., Oliveira, C.C., Acco, A., Nader, H.B., Chammas, R., Iacomini, M., Franco, C.R.C., Trindade, E.S.
Source:
Carbohydrate polymers 2017 v.178 pp. 95-104
ISSN:
0144-8617
Subject:
Pleurotus eryngii, basidiomata, body weight, hydroxyl radicals, melanoma, methylation, mice, models, mushrooms, nuclear magnetic resonance spectroscopy, phenotype, therapeutics, toxicity
Abstract:
A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii (“King Oyster”) basidiocarps and its biological properties were evaluated. Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found. Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells. MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion. Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control. Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed. Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
Agid:
5823211