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Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii

Biscaia, S.M.P., Carbonero, E.R., Bellan, D.L., Borges, B.S., Costa, C.R., Rossi, G.R., Gonçalves, J.P., Melo, C.M., Lívero, F.A.R., Ruthes, A.C., Zotz, R., Silva, E.V., Oliveira, C.C., Acco, A., Nader, H.B., Chammas, R., Iacomini, M., Franco, C.R.C., Trindade, E.S.
Carbohydrate polymers 2017 v.178 pp. 95-104
Pleurotus eryngii, basidiomata, body weight, hydroxyl radicals, melanoma, methylation, mice, models, mushrooms, nuclear magnetic resonance spectroscopy, phenotype, therapeutics, toxicity
A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii (“King Oyster”) basidiocarps and its biological properties were evaluated. Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found. Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells. MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion. Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control. Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed. Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.