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Hypoglycemic effect and mechanism of a pectic polysaccharide with hexenuronic acid from the fruits of Ficus pumila L. in C57BL/KsJ db/db mice

Wu, Jianjun, Chen, Miaomiao, Shi, Songshan, Wang, Huijun, Li, Ning, Su, Juan, Liu, Ruimin, Huang, Zhenlin, Jin, Hong, Ji, Xueqing, Wang, Shunchun
Carbohydrate polymers 2017 v.178 pp. 209-220
Ficus pumila, Fourier transform infrared spectroscopy, alcohols, blood glucose, boiling, esterification, fruit acids, fruits, functional foods, gas chromatography-mass spectrometry, gel chromatography, glucokinase, glucose-6-phosphatase, glycemic control, glycemic effect, glycogen, glycogenesis, glycogenolysis, hyperglycemia, insulin, methylation, mice, nuclear magnetic resonance spectroscopy, signal transduction
In this study, a particular pectic polysaccharide (FPLP) was extracted and purified from the fruits of Ficus pumila Linn. through boiling water extraction, alcohol precipitation, diethylaminoethyl-Sepharose Fast Flow chromatography and Superdex™ G-75 gel filtration chromatography. Analysis of high-performance gel permeation chromatography, FTIR, GC–MS, methylation and 1D/2D NMR spectroscopy revealed that FPLP (Mw: 34.69kDa) is a linear (1,4)-α-d-galacturonic acid binding 1.30% branched chain hexenuronic acid with 23.34% methyl esterification. Treatment with FPLP ameliorated hyperglycaemia in association with an improvement in hepatic glycogen metabolism in C57BL/KsJ db/db mice. The activation of IRS-1/PI3K/Akt/GSK3β/GS insulin signalling pathway and AMPK/GSK3β/GS signalling pathway and the regulation of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase expressions involved in hepatic glycogenesis and glycogenolysis were considered the therapeutic mechanisms of FPLP. These results provide a new insight for investigating the effects of pectic polysaccharides on blood glucose control and suggest that FPLP is a promising nutraceutical for treatment of T2DM.