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Localization of 'Candidatus Liberibacter solancearum' (Rhizobiales: Rhizobiaceae) in Bactericera cockerelli (Hemiptera: Triozidae)

Author:
Cooper, W. Rodney, Sengoda, Venkatesan G., Munyaneza, Joseph E.
Source:
Annals of the entomological society of america 2014 v.107 no.1 pp. 204-210
ISSN:
1938-2901
Subject:
plant pathogenic bacteria, Solanum tuberosum, insect vectors, hemolymph, potatoes, dieback, tomatoes, fluorescence in situ hybridization, Bactericera cockerelli, digestive tract, nymphs, Capsicum, Triozidae, imagos, Candidatus Liberibacter, host plants, zebra chip disease, Rhizobiaceae, quantitative polymerase chain reaction, host-pathogen relationships, instars, disease transmission, salivary glands, pepper
Abstract:
Candidatus Liberibacter solanacearum is a pathogen of solanaceous crops (Solanales: Solanaceae) that causes zebra chip disease of potato (Solanum tuberosum L.) and plant dieback in tomato (S. lycopersicum L.) and pepper (Capsicum spp.). This pathogen is vectored by the potato/ tomato psyllid Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), but little is known about the interactions between B. cockerelli and Ca. Liberibacter solanacearum. Fluorescence in situ hybridization was used to assess the incidence of Ca. Liberibacter solanacearum in the hemolymph, bacteriomes, alimentary canals, and salivary glands of B. cockerelli. Liberibacter was observed in 66% of alimentary canals, 39% of salivary glands, and 40% of bacteriomes dissected from adult psyllids. Compared with adults, the organs of fifth instars appeared less likely to harbor Liberibacter, which was observed in 52% of alimentary canals, 10% of salivary glands, and 6% of bacteriomes dissected from the nymphs. Results of real-time polymerase chain reaction conÞrmed that fewer Þfth instars were infected with Liberibacter compared with adults and indicated that Þfth instars were less likely to transmit the pathogen to noninfected host plants. These observations of the localization of Ca. Liberibacter solanacearum in the organs and tissues of B. cockerelli adults and nymphs will aid the study of Liberibacter-psyllid interactions and the epidemiology of Ca. Liberibacter solanacearum.
Agid:
58248
Handle:
10113/58248