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Production of bioethanol from multiple waste streams of rice milling
- Favaro, Lorenzo, Cagnin, Lorenzo, Basaglia, Marina, Pizzocchero, Valentino, van Zyl, Willem Heber, Casella, Sergio
- Bioresource technology 2017 v.244 pp. 151-159
- amylases, bioethanol, bioprocessing, ethanol, ethanol production, feedstocks, fermentation, fungi, genetically engineered microorganisms, lignocellulose, milling, milling byproducts, rice, rice bran, rice hulls, saccharification
- This work describes the feasibility of using rice milling by-products as feedstock for bioethanol. Starch-rich residues (rice bran, broken, unripe and discolored rice) were individually fermented (20%w/v) through Consolidated Bioprocessing by two industrial engineered yeast secreting fungal amylases. Rice husk (20%w/v), mainly composed by lignocellulose, was pre-treated at 55°C with alkaline peroxide, saccharified through optimized dosages of commercial enzymes (Cellic® CTec2) and fermented by the recombinant strains.Finally, a blend of all the rice by-products, formulated as a mixture (20%w/v) according to their proportions at milling plants, were co-processed to ethanol by optimized pre-treatment, saccharification and fermentation by amylolytic strains.Fermenting efficiency for each by-product was high (above 88% of the theoretical) and further confirmed on the blend of residues (nearly 52g/L ethanol). These results demonstrated for the first time that the co-conversion of multiple waste streams is a promising option for second generation ethanol production.