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Isolation and characterization of microsatellite markers for Viola mirabilis (Violaceae) using a next‐generation sequencing platform
- Kang, Jong‐Soo, Lee, Byoungyoon, Kwak, Myounghai
- Plant species biology 2017 v.32 no.4 pp. 448-454
- Viola, alleles, endangered species, genetic markers, heterozygosity, high-throughput nucleotide sequencing, loci, microsatellite repeats, phylogeography, population genetics, South Korea
- We isolated and characterized microsatellite loci in Viola mirabilis (Violaceae), an endangered species from South Korea. Twenty‐three polymorphic microsatellite loci were developed and tested in Korean, Chinese and Japanese populations. The number of alleles per locus varied from two to eight. The observed and expected heterozygosities within the three populations were 0.000–0.625 and 0.469–0.695, respectively. A total of six loci in the Korean population, one locus in the Chinese population and seven loci in the Japanese population deviated from Hardy–Weinberg equilibrium. We expect that these newly developed microsatellite markers will contribute to understanding the phylogeography and population genetics of V. mirabilis, which will aid in developing conservation strategies for this species.