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Cytotoxic Cardiac Glycoside from the Parasitic Plant Cuscuta reflexa

Author:
Muhammad Ali Versiani, Asma Kanwal, Shaheen Faizi, Ahsana Dar Farooq
Source:
Chemistry of natural compounds 2017 v.53 no.5 pp. 915-922
ISSN:
0009-3130
Subject:
Cuscuta reflexa, anticarcinogenic activity, beta-sitosterol, bioassays, chemical structure, coumarins, cytotoxicity, fractionation, glucosides, nuclear magnetic resonance spectroscopy, parasitic plants, prostatic neoplasms, spectral analysis, stable isotopes, ursolic acid
Abstract:
Bioassay guided fractionation of the cytotoxic extract of the plant Cuscuta reflexa, employing cytotoxic assay against a panel of 60 cell lines, led to the isolation of 13 compounds identified as odoroside H (1), 21-hydroxyodoroside H (2), neritaloside (3), strospeside (4), 16-β-hydroxydigitoxin (5), N-trans and cis feruloyl tyramines (6 and 7), ethyl caffeate (8), coumarins (9 and 10), ursolic acid (11), β-sitosterol glucoside (12), and 4-O-p-coumaroyl-β-D-glucoside (13). Compound 2 was found to be a new natural product while 1, 3, 4, 5, 6, 7, 10, and 11 are known compounds isolated from this source for the first time. Compounds 1, 3, and 4 possessed very good cytotoxic activity against renal and prostate cancer cell lines. Of these compounds, 1 showed a positive hollow fiber test and was selected for in vivo xenograft testing. The structures of compounds 1–5 have been established through chemical and spectral studies including UV, IR, mass, NMR (¹H and ¹³C), and 2D NMR (COSY-45°, NOESY, HMQC, HMBC, and J-resolved) experiments.
Agid:
5827523