U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Cytotoxic Cardiac Glycoside from the Parasitic Plant Cuscuta reflexa

Muhammad Ali Versiani, Asma Kanwal, Shaheen Faizi, Ahsana Dar Farooq
Chemistry of natural compounds 2017 v.53 no.5 pp. 915-922
Cuscuta reflexa, anticarcinogenic activity, beta-sitosterol, bioassays, chemical structure, coumarins, cytotoxicity, fractionation, glucosides, nuclear magnetic resonance spectroscopy, parasitic plants, prostatic neoplasms, spectral analysis, stable isotopes, ursolic acid
Bioassay guided fractionation of the cytotoxic extract of the plant Cuscuta reflexa, employing cytotoxic assay against a panel of 60 cell lines, led to the isolation of 13 compounds identified as odoroside H (1), 21-hydroxyodoroside H (2), neritaloside (3), strospeside (4), 16-β-hydroxydigitoxin (5), N-trans and cis feruloyl tyramines (6 and 7), ethyl caffeate (8), coumarins (9 and 10), ursolic acid (11), β-sitosterol glucoside (12), and 4-O-p-coumaroyl-β-D-glucoside (13). Compound 2 was found to be a new natural product while 1, 3, 4, 5, 6, 7, 10, and 11 are known compounds isolated from this source for the first time. Compounds 1, 3, and 4 possessed very good cytotoxic activity against renal and prostate cancer cell lines. Of these compounds, 1 showed a positive hollow fiber test and was selected for in vivo xenograft testing. The structures of compounds 1–5 have been established through chemical and spectral studies including UV, IR, mass, NMR (¹H and ¹³C), and 2D NMR (COSY-45°, NOESY, HMQC, HMBC, and J-resolved) experiments.