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A high-throughput multiplex tandem PCR assay for the screening of genetically modified maize
- Wei, Shuang, Wang, Chenguang, Zhu, Pengyu, Zhou, Guangbiao, Fu, Wei, Wu, Xiyang
- Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.87 pp. 169-176
- DNA, corn, feeds, foods, genes, polymerase chain reaction, screening, transgenic plants
- Genetically modified organisms (GMO) are increasingly gaining acceptance, which has led to an increasing demand for high-throughput methods for their detection. This study describes development of a high-throughput and low-cost multiplex tandem PCR (MT-PCR) assay for the screening of transgenic maize of 14 targets, containing 7 genetically modified (GM) common screening elements, 6 transgenic maize events and a maize reference gene, using SYBR Green I for quantification. The 14-target high-throughput multiplex tandem PCR assay successfully amplified products from single-event samples with a GM maize DNA amount less to 0.001 ng, and displayed high specificity. This assay can efficiently be used for screening GM maize in foods and feeds.