Main content area

Callus induction and establishment of cell suspension cultures of the halophyte Armeria maritima (Mill.) Willd.

Gourguillon, L., Rustenholz, C., Lobstein, A., Gondet, L.
Scientia horticulturae 2018 v.233 pp. 407-411
Armeria maritima, callus, callus formation, cell suspension culture, cotyledons, explants, halophytes, horticulture, leaves, mature plants, roots, secondary metabolites, seedlings, seeds
The objective of this work was to establish a cell suspension culture from Armeria maritima (Mill.) Willd., an halophyte so far only valuable for horticultural purposes, as a pre-requisite step towards the production of bio-actives secondary metabolites in vitro. For the initiation of callogenesis, five types of explants were tested: leaves from mature plants; leaves, cotyledons and roots from axenic seedlings; seeds. Three criteria either from a quantitative (percentage of callus induction) or a qualitative basis (aspect and friability) were used to evaluate the effectiveness of callus formation. Seeds-derived calli grown in the dark on MS medium supplemented with 4.5 μM 2.4D and 0.93 μM KIN were successfully selected for the establishment of cell suspension cultures, characterized by a growth index of 3.4 after 14 days of culture.