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Morinda citrifolia lipid transfer protein 1 exhibits anti-inflammatory activity by modulation of pro- and anti-inflammatory cytokines

Campos, Dyély C.O., Costa, Andrea S., Luz, Patrícia B., Soares, Pedro M.G., Alencar, Nylane M.N., Oliveira, Hermógenes D.
International journal of biological macromolecules 2017 v.103 pp. 1121-1129
Morinda citrifolia, amylases, anti-inflammatory activity, carboxylic ester hydrolases, carrageenan, cell movement, dextran, edema, interleukin-10, interleukin-1beta, interleukin-6, lipid transfer proteins, mice, models, peritonitis, seeds, sodium chloride, solubilization, thermal stability, tumor necrosis factor-alpha
Previous reports have demonstrated that a thermostable lipid transfer protein isolated from noni seeds (McLTP1; 9.4kDa) displays anti-nociceptive and anti-inflammatory activities. This work aimed to investigate the underlying mechanisms of the anti-inflammatory activity of McLTP1 in mice. The protein was solubilised in sterile saline (0.9% NaCl) immediately before the treatment of mice by oral or intraperitoneal routes at doses of 8mg/kg. Given orally or intraperitoneally, McLTP1 significantly inhibited (p<0.05) cell migration in experimental models of carrageenan-induced peritonitis and the formation of paw oedema induced by carrageenan and dextran. Additionally, McLTP1 demonstrated the ability to significantly inhibit the production of the cytokines IL-1β, IL-6, and TNF-α (p<0.05) and to promote an increase in the production of the anti-inflammatory cytokine IL-10. The treatment of mice with McLTP1 by the oral or i.p route reduced pancreatic injury and activities of amylase, lipase, and pancreatitis-associated lung injury. This study suggested that the observed anti-inflammatory effects of McLTP1 can be related to modulation of pro- and anti-inflammatory cytokine levels.