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Capillary electrophoresis – Mass spectrometry metabolomics analysis revealed enrichment of hypotaurine in rat glioma tissues

Author:
Gao, Peng, Ji, Min, Fang, Xueyan, Liu, Yingyang, Yu, Zhigang, Cao, Yunfeng, Sun, Aijun, Zhao, Liang, Zhang, Yong
Source:
Analytical biochemistry 2017 v.537 pp. 1-7
ISSN:
0003-2697
Subject:
brain, capillary electrophoresis, cell culture, chemical elements, enzyme activity, humans, immunocytochemistry, mass spectrometry, metabolites, metabolome, metabolomics, rats, staining, tissues
Abstract:
Glioma is one of the most lethal brain malignancies with unknown etiologies. Many metabolomics analysis aiming at diverse kinds of samples had been performed. Due to the varied adopted analytical platforms, the reported disease-related metabolites were not consistent across different studies. Comparable metabolomics results are more likely to be acquired by analyzing the same sample types with identical analytical platform. For tumor researches, tissue samples metabolomics analysis own the unique advantage that it can gain more direct insight into disease-specific pathological molecules. In this light, a previous reported capillary electrophoresis – mass spectrometry human tissues metabolomics analysis method was employed to profile the metabolome of rat C6 cell implantation gliomas and the corresponding precancerous tissues. It was found that 9 metabolites increased in the glioma tissues. Of them, hypotaurine was the only metabolite that enriched in the malignant tissues as what had been reported in the relevant human tissues metabolomics analysis. Furthermore, hypotaurine was also proved to inhibit α-ketoglutarate-dependent dioxygenases (2-KDDs) through immunocytochemistry staining and in vitro enzymatic activity assays by using C6 cell cultures. This study reinforced the previous conclusion that hypotaurine acted as a competitive inhibitor of 2-KDDs and proved the value of metabolomics in oncology studies.
Agid:
5830846