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Effects of flow velocity on growth, food intake, body composition, and related gene expression of Haliotis discus hannai Ino

Author:
Xiaolong, Gao, Xian, Li, Mo, Zhang, Fucun, Wu, Ce, Shi, Ying, Liu
Source:
Aquaculture 2017 v.481 pp. 48-57
ISSN:
0044-8486
Subject:
Haliotis discus hannai, abalone, alpha-amylase, ammonium nitrogen, aquaculture, body composition, body weight, endo-1,4-beta-glucanase, energy, enzyme activity, food conversion, food intake, hexokinase, lactic acid, nitrite nitrogen, pepsin, protein content, pyruvate kinase, specific growth rate, water content, water flow, water quality
Abstract:
This study investigated the effects of different flow velocities in a circulating aquaculture system on growth, food intake, and related gene expression of the abalone Haliotis discus hannai Ino. Abalones (shell length: 41.39±2.85mm, body weight: 8.19±0.66g) were cultured at three flow velocities; high-velocity (400L/h), medium-velocity (300L/h), and low-velocity (200L/h) during the course of the experiment. Four repeats of each flow velocity were conducted over an experimental cycle of 90days. Results showed that the survival and specific growth rate of abalones in the 200L/h group were significantly lower than in any other group at the end of the experiment, while total ammonia nitrogen and NO2‑N concentration in the water was significantly higher than that in any other group (P<0.05). Food intake, food conversion efficiency, protein content, pepsin, and α-amylase activity of abalones in the 300L/h group were significantly higher than in the 200 and 400L/h groups (P<0.05), but there was no significant difference identified between 200 and 400L/h groups (P>0.05). Although no significant difference was identified between 300 and 400L/h groups with respect to cellulase activity or the expression levels of Hdaly, both were significantly higher than in the 200L/h group (P<0.05). In the 400L/h group, hexokinase and pyruvate kinase activity, and lactic acid content were significantly higher compared with in the 300L/h group (P<0.05). The ash and fat contents of abalones in the 200L/h group were significantly lower than in any other group, but moisture content was significantly higher (P<0.05). At Day 90, the expression levels of Hdamyl, Hdlam, and Hdcel in the 300L/h group were significantly higher than in any other group (P<0.05), and compared with Day 45, the expression levels of were Hdamyl significantly increased (P<0.05). Although no significant difference was identified between 200 and 400L/h groups with respect to the expression levels of Mn-SOD and CAT, both were significantly higher than those in the 300L/h group (P<0.05). Therefore, control of flow velocity at 300L/h will not only stimulate the food intake and growth of abalones, but also reduce energy consumption to resist against water flow impact and avoid oxidative damage due to water quality deterioration. This will be beneficial for abalone health and will improve aquaculture production.
Agid:
5831300