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Use of Polymerase Chain Reaction in Detection of Marek's Disease and Reticuloendotheliosis Viruses in Formalin-Fixed, Paraffin-Embedded Tumorous Tissues.

Weisheng Cao, Jody Mays, John Dunn, Richard Fulton, Robert Silva, Aly Fadly
Avian diseases 2013 v.57 no. pp. 785-789
chickens, immunohistochemistry, polymerase chain reaction, reticuloendotheliosis, viruses, Gallid alphaherpesvirus 2, formalin, Reticuloendotheliosis virus, disease diagnosis
A simple polymerase chain reaction (PCR) method was developed for the diagnosis of Marek’s disease (MD) and reticuloendotheliosis (RE) in formalin-fixed paraffin-embedded (FFPE) tissues; and for the diagnosis of MD in tissues only preserved in 10% neutral buffered formalin. MD virus (MDV) and RE virus (REV) were detected in FFPE tissues stored for over 20 years. MDV was also detected in tissues only preserved in formalin for up to 6 months. The data indicate that PCR of formalin fixed (FF) and FFPE tissues is a simple and valuable tool that can be used to identify MD and RE infection. The method described in this paper is a good alternative to any biological, molecular, or immunohistochemical assay to confirm the diagnosis of MD and RE, as it does not require shipping frozen tissues to diagnostic laboratory.