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Selection and Application of Strand Displacement Probes for a Fumonisin B1 Aptamer
- Hong-qi Wang, Jun-yan Wang, Hui-jie Hong, Hai-yan Yin, Chris Maragos, Ling Zhang, Ji-hong Liu
- Quality and Safety of Agro-Products 2017 v.1 no. pp. 44-48
- DNA probes, Fusarium verticillioides, corn, fluorescence, food contamination, fumonisin B1, microbial contamination, oligonucleotides
- Fumonisin B1 (FB1) is a toxin produced by Fusarium moniliforme, mainly on contaminated maize and maize products. In this study a solid surface chain displacement strategy was used to isolate oligonucleotide displacement probes for a FB1 aptamer. The probes were used as the basis for the development of a fluorescence polarization detection method for FB1 in corn. In actual samples the dynamic response range was 10 nm to 200 nM. The method is a homogeneous detection technology. It has low cost, good reproducibility, is easy to operate, and can be used to process a large number of samples for parallel detection.