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Selection and Application of Strand Displacement Probes for a Fumonisin B1 Aptamer

Author:
Wang, Hong-qi, Wang, Jun-yan, Hong, Hui-jie, Yin, Hai-yan, Maragos, Chris, Zhang, Ling, Liu, Ji-hong
Source:
Quality and Safety of Agro-Products 2017 v.1 pp. 44-48
ISSN:
1674-8255
Subject:
DNA probes, Fusarium verticillioides, corn, fluorescence, food contamination, fumonisin B1, microbial contamination, oligonucleotides
Abstract:
Fumonisin B1 (FB1) is a toxin produced by Fusarium moniliforme, mainly on contaminated maize and maize products. In this study a solid surface chain displacement strategy was used to isolate oligonucleotide displacement probes for a FB1 aptamer. The probes were used as the basis for the development of a fluorescence polarization detection method for FB1 in corn. In actual samples the dynamic response range was 10 nm to 200 nM. The method is a homogeneous detection technology. It has low cost, good reproducibility, is easy to operate, and can be used to process a large number of samples for parallel detection.
Agid:
5832874
Handle:
10113/5832874