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Rethinking cell-cycle-dependent gene expression in Schizosaccharomyces pombe

Author:
Cooper, Stephen
Source:
Antonie van Leeuwenhoek 2017 v.110 no.11 pp. 1485-1491
ISSN:
0003-6072
Subject:
Schizosaccharomyces pombe, cell cycle, gene expression, genes
Abstract:
Three studies of gene expression during the division cycle of Schizosaccharomyces pombe led to the proposal that a large number of genes are expressed at particular times during the S. pombe cell cycle. Yet only a small fraction of genes proposed to be expressed in a cell-cycle-dependent manner are reproducible in all three published studies. In addition to reproducibility problems, questions about expression amplitudes, cell-cycle timing of expression, synchronization artifacts, and the problem with methods for synchronizing cells must be considered. These problems and complications prompt the idea that caution should be used before accepting the conclusion that there are a large number of genes expressed in a cell-cycle-dependent manner in S. pombe.
Agid:
5835568