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Prolyl isomerase Pin1 promotes proplatelet formation of megakaryocytes via tau

Author:
Shimizu, Taiki, Uchida, Chiyoko, Shimizu, Ritsuko, Motohashi, Hozumi, Uchida, Takafumi
Source:
Biochemical and biophysical research communications 2017 v.493 pp. 946-951
ISSN:
0006-291X
Subject:
cell lines, genes, isomerization, megakaryocytes, mice, microtubules, polymerization
Abstract:
Here we show that Pin1, a peptidyl-prolyl cis/trans isomerase which catalyzes the isomerization of phosphorylated Ser/Thr-Pro, is a regulatory molecule of thrombopoiesis. We found that mice lacking the Pin1 gene (Pin1−⁄− mice) formed more megakaryocytes (MKs) than wild type mice (WT mice), and that the proplatelet formation of MKs was poorer in Pin1−⁄− mice than WT mice. Treatment of Meg-01 cells, a megakaryoblastic floating cell line, with shRNA against Pin1 suppressed the proplatelet formation. Expression of tau, a microtubule associated protein was induced in MKs during proplatelet formation. Pin1 bound tau and promoted microtubule polymerization. Our results show that Pin1 serves as a positive regulatory molecule of proplatelet formation of MKs by enhancing the function of phosphorylated tau.
Agid:
5836859