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Modification of the cellular antioxidant activity (CAA) assay to study phenolic antioxidants in a Caco-2 cell line

Author:
Kellett, Mary E., Greenspan, Phillip, Pegg, Ronald B.
Source:
Food chemistry 2018 v.244 pp. 359-363
ISSN:
0308-8146
Subject:
antioxidant activity, antioxidants, fluorescence, foods, human cell lines, in vitro studies, phenolic compounds, physiological transport, quercetin
Abstract:
In vitro assays are widely used to analyze the antioxidant potential of compounds, but they cannot accurately predict antioxidant behavior in living systems. Cell-based assays, like the cellular antioxidant activity (CAA) assay, are gaining importance as they provide a biological perspective. When the CAA assay was employed to study phenolic antioxidants using hepatocarcinoma (HepG2) cells, quercetin showed antioxidant activity in HepG2 cells; 25 and 250μM quercetin reduced fluorescence by 17.1±0.9% and 58.6±2.4%, respectively. (+)-Catechin, a phenolic antioxidant present in many foods, bestowed virtually no CAA in HepG2 cells. When Caco-2 cells were employed, more robust antioxidant activity was observed; 50μM (+)-catechin and quercetin reduced fluorescence by 54.1±1.4% and 63.6±0.9%, respectively. Based on these results, likely due to differences in active membrane transport between the cell types, the Caco-2-based CAA assay appears to be a more appropriate method for the study of certain dietary phenolics.
Agid:
5838849