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Two CAPS markers predict Verticillium wilt resistance in wild Solanum species

Uribe, Pedro, Jansky, Shelley, Halterman, Dennis
Molecular breeding 2014 v.33 no.2 pp. 465-476
Solanum tuberosum, Verticillium dahliae, Verticillium wilt, diploidy, fungi, genes, genetic markers, marker-assisted selection, pathogens, potatoes, tomatoes, wilting
Verticillium wilt of potato is a persistent problem across several production areas of the United States. The disease, which is caused primarily by the fungus Verticillium dahliae is difficult to manage, causes yield losses and contaminates the soils for subsequent plantings. Control strategies based on host resistance are seen as long term, stable solutions, but difficult to achieve given the genetic nature of the host. To provide breeders with marker assisted selection alternatives for this disease, we generated a molecular marker on the coding region of Ve2, a potato gene with homology to the tomato Ve2 gene that confers resistance to V. dahliae. The position for the marker was determined according to the consensus sequences of Ve2 homologs of wild Solanum species that were screened for resistance or susceptibility to the pathogen. Experimentation leading to the development of the marker and tests of its usefulness against a wide range of Solanum tuberosum diploid and tetraploid material are presented here.