Main content area

Effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants Solanum lycopersicum and Brassica oleracea var. botrytis

Singh, Ajey, Singh, N.B., Hussain, Imtiyaz, Singh, Himani
Journal of biotechnology 2017 v.262 pp. 11-27
Brassica oleracea var. botrytis, Fourier transform infrared spectroscopy, Morus alba, Solanum lycopersicum, X-ray diffraction, antioxidant activity, atomic absorption spectrometry, bioaccumulation, cauliflower, chlorophyll, crops, cupric oxide, dose response, electrolytes, environmental impact, enzyme activity, hydrogen peroxide, leaf extracts, leaves, lignin, lipid peroxidation, metabolism, nanoparticles, nitroblue tetrazolium, oxidants, reducing agents, roots, seedlings, sugar content, tomatoes, transmission electron microscopes, transmission electron microscopy
Study on the ecological effect of metal oxide nanomaterials (NMs) has quickly amplified over the precedent years because it is assumed that these NMs will sooner or later be released into the environment. The present study deals with biologically oriented process for the green synthesis of copper oxide nanoparticles (CuO NPs) by using Morus alba leaf extract as reducing agent. Powder X-ray diffraction (XRD) and transmission electron microscope (TEM) analysis revealed the monoclinic phase and 20–40nm size respectively. The presence of reducing and capping agents revealed by Fourier transform infrared (FTIR) spectroscopy. The seedlings of Brassica oleracea var. botrytis and Solanum lycopersicum were exposed to 10, 50, 100, and 500mgL−1 concentrations of CuO NPs in the sand medium. Bioaccumulation of Cu was also investigated by atomic absorption spectroscopy (AAS). Plant exposure to 100 and 500mgL−1 of CuO NPs has resulted in significant reduction of total chlorophyll and sugar content in the two test plants while 10mgL−1 of NPs slightly increased the pigment and sugar content in tomato plants only. Augmentation of lipid peroxidation, electrolyte leakage, and antioxidant enzyme activity was observed in a dose dependent manner upon plants exposure to CuO NPs. Deposition of lignin in roots of both plants treated with the highest concentration of CuO NPs was observed. Histochemical analysis of leaves of treated plant with nitroblue tetrazolium and 3′3′ diaminobenzidine showed a concentration dependent increase in superoxide and hydrogen peroxide formation in leaves. The green synthesis of CuO NPs was carried out by using Morus alba leaf extract. Accumulation of NPs more actively by tomato plants as compared to cauliflower was possibly due to the difference in root morphology. The histochemical visualization highlights the spatial organization of oxidant biochemistry occurring in response to metal stress.