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Antimicrobial biodegradable food packaging impregnated with Bacteriocin 7293 for control of pathogenic bacteria in pangasius fish fillets

Author:
Woraprayote, Weerapong, Pumpuang, Laphaslada, Tosukhowong, Amonlaya, Zendo, Takeshi, Sonomoto, Kenji, Benjakul, Soottawat, Visessanguan, Wonnop
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.89 pp. 427-433
ISSN:
0023-6438
Subject:
Aeromonas hydrophila, Escherichia coli, Gram-negative bacteria, Listeria monocytogenes, Pangasius, Pseudomonas aeruginosa, Salmonella Typhimurium, Staphylococcus aureus, Weissella hellenica, antimicrobial packaging, bacteriocins, biocomposites, biodegradability, fish fillets, food packaging, freshwater fish, iso-octanes, pathogens, polylactic acid
Abstract:
This study aimed to develop an antimicrobial biodegradable food packaging for control of pathogens in pangasius fish fillets, one of the world's most consumed freshwater fish. Bacteriocin 7293 (Bac7293), a novel bacteriocin from Weissella hellenica BCC 7293, was diffusion coated onto poly (lactic acid)/sawdust particle biocomposite film (PLA/SP) with the maximum amount of 19.54 ± 2.87 μg/cm2. According to the JIS Z 2801:2000 standard testing method, the produced PLA/SP film impregnated with Bac7293 (PLA/SP + Bac7293) effectively inhibited both Gram-positive (Listeria monocytogenes and Staphylococcus aureus) and Gram-negative bacteria (Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli and Salmonella Typhimurium) which have been considered as a reason for the rejection of pangasius fish fillets. This antimicrobial film inhibited the growth of target microorganisms by about 2–5 log CFU/cm2, depended on bacterial strains, compared with control, both in vitro and in pangasius fish fillets by means of challenge test. The overall migration of PLA/SP + Bac7293 film to food simulants was maximized in isooctane (3.67 ± 0.47 mg/dm2) which was much lower than the overall migration limit regulated by the commission of the European communities. This suggested high potential application of PLA/SP + Bac7293 as a good antimicrobial packaging for pangasius fish fillets.