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The effect of silver or gallium doped titanium against the multidrug resistant Acinetobacter baumannii

Author:
Cochis, A., Azzimonti, B., Della Valle, C., De Giglio, E., Bloise, N., Visai, L., Cometa, S., Rimondini, L., Chiesa, R.
Source:
Biomaterials 2016 v.80 pp. 80-95
ISSN:
0142-9612
Subject:
Acinetobacter baumannii, alkaline phosphatase, antibacterial properties, bacteria, bacterial adhesion, biocompatible materials, biofilm, electrochemistry, fracture fixation, gallium, multiple drug resistance, pathogens, porosity, scanning electron microscopy, silver, titanium, viability
Abstract:
Implant-related infection of biomaterials is one of the main causes of arthroplasty and osteosynthesis failure. Bacteria, such as the rapidly-emerging Multi Drug Resistant (MDR) pathogen Acinetobacter Baumannii, initiate the infection by adhering to biomaterials and forming a biofilm. Since the implant surface plays a crucial role in early bacterial adhesion phases, titanium was electrochemically modified by an Anodic Spark Deposition (ASD) treatment, developed previously and thought to provide osseo-integrative properties. In this study, the treatment was modified to insert gallium or silver onto the titanium surface, to provide antibacterial properties.The material was characterized morphologically, chemically, and mechanically; biological properties were investigated by direct cytocompatibility assay, Alkaline Phosphatase (ALP) activity, Scanning Electron Microscopy (SEM), and Immunofluorescent (IF) analysis; antibacterial activity was determined by counting Colony Forming Units, and viability assay.The various ASD-treated surfaces showed similar morphology, micrometric pore size, and uniform pore distribution. Of the treatments studied, gallium-doped specimens showed the best ALP synthesis and antibacterial properties.This study demonstrates the possibility of successfully doping the surface of titanium with gallium or silver, using the ASD technique; this approach can provide antibacterial properties and maintain high osseo-integrative potential.
Agid:
5843341