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Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Joo, Jeong Chan, Oh, Young Hoon, Yu, Ju Hyun, Hyun, Sung Min, Khang, Tae Uk, Kang, Kyoung Hee, Song, Bong Keun, Park, Kyungmoon, Oh, Min-Kyu, Lee, Sang Yup, Park, Si Jae
Bioresource technology 2017 v.245 pp. 1692-1700
Corynebacterium glutamicum, Miscanthus, Pseudomonas putida, batch fermentation, biosynthesis, cell viability, feedstocks, genes, glucose, hydrolysates, hydrolysis, lysine
This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.