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Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process
- Joo, Jeong Chan, Oh, Young Hoon, Yu, Ju Hyun, Hyun, Sung Min, Khang, Tae Uk, Kang, Kyoung Hee, Song, Bong Keun, Park, Kyungmoon, Oh, Min-Kyu, Lee, Sang Yup, Park, Si Jae
- Bioresource technology 2017 v.245 pp. 1692-1700
- Corynebacterium glutamicum, Miscanthus, Pseudomonas putida, batch fermentation, biosynthesis, cell viability, feedstocks, genes, glucose, hydrolysates, hydrolysis, lysine
- This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.