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The first genetic map of the American cranberry: exploration of synteny conservation and quantitative trait loci

Georgi, Laura, Johnson-Cicalese, Jennifer, Honig, Josh, Das, Sushma Parankush, Rajah, Veeran D., Bhattacharya, Debashish, Bassil, Nahla, Rowland, Lisa J., Polashock, James, Vorsa, Nicholi
Theoretical and applied genetics 2013 v.126 pp. 673
Arabidopsis thaliana, Vaccinium macrocarpon, Vitis vinifera, biosynthesis, cultivars, flavonoids, fruit yield, genes, grapes, linkage groups, loci, microsatellite repeats, pathogens, pleiotropy, quantitative trait loci, titratable acidity
The first genetic map of cranberry has been constructed, comprising 14 linkage groups totaling 879.9 cM with an estimated coverage of 82.2%. This map, based on four mapping populations segregating for field fruit rot resistance, contains 136 distinct loci. Mapped markers include blueberry simple sequence repeat (SSR) and cranberry sequence-characterized amplified region (SCAR) markers previously used for fingerprinting cranberry cultivars. In addition, SSR markers were developed near cranberry sequences resembling genes involved in flavonoid biosynthesis or defense against necrotrophic pathogens, or conserved orthologous set (COS) sequences. The cranberry SSRs were developed from next-generation cranberry genomic sequence assemblies; thus, the positions of these SSRs on the genomic map provide information about the genomic location of the sequence scaffold from which they were derived. The use of SSR markers near COS and other functional sequences, plus 33 SSR markers from blueberry, facilitates comparisons of this map with maps of other plant species. Regions of the cranberry map were identified that showed conservation of synteny with grape and Arabidopsis thaliana. Positioned on this map are quantitative trait loci (QTL) for field fruit rot resistance (FFRR), fruit weight, titratable acidity, and sound fruit yield. The sound fruit yield QTL is adjacent to one of the fruit weight QTL and may reflect pleiotropy. Two of the FFRR QTL are in regions of conserved synteny with grape and span defense gene markers, and the third FFRR QTL spans a flavonoid biosynthetic gene.