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Precision Parameters for AOAC Bacillus stearothermophilus Disc Assay Based on FDA Milk Laboratory Quality Assurance 1982–1986 Samples

Author:
Peeler, J. T., Messer, J. W., Houghtby, G. A., Leslie, J. E., Barnett, J. E.
Source:
Journal of food protection 1989 v.52 no.12 pp. 867-870
ISSN:
0362-028X
Subject:
Geobacillus stearothermophilus, agar, ampicillin, cephapirin, erythromycin, milk, neomycin, quality control, statistical analysis
Abstract:
Inhibitory substance (antibiotic) test results from State Split Milk Samples were used to estimate precision parameters and to compare antibiotic medium 4 (A4) and PM indicator (PM) agars. Five inhibitory substances (ampicillin, cephapirin, erythromycin, neomycin, and penicillin-G) were tested. Repeatability relative standard deviations (RSDr) ranged from 1.0 to 4.8%, and the reproducibility relative standard deviations (RSDR) ranged from 4.8 to 10.4%. Zone sizes of erythromycin, neomycin, and penicillin-G were significantly larger on PM agar (α = 0.05) than on A4 agar. The reverse was observed for cephapirin. No difference between agars was noted for ampicillin.
Agid:
5850138