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Quantitative Comparison of Two Enrichment Methods for Isolating Listeria monocytogenes from Inoculated Ice Cream

Author:
Hitchins, Anthony D.
Source:
Journal of food protection 1989 v.52 no.12 pp. 898-900
ISSN:
0362-028X
Subject:
Food and Drug Administration, Listeria monocytogenes, USDA, alkali treatment, ice cream, portion size
Abstract:
Two enrichment methods for isolating Listeria monocytogenes (the Lovett method, formerly used by the U.S. Food and Drug Administration, and the revised method for the U. S. Department of Agriculture) were compared using 25 g test portions of spiked vanilla ice cream. Both methods were found to be equally sensitive. Prolonging the enrichments to 7 d and the use of alkali pretreatment had no significant effect on the results. Reduction of the test portion size from 25 to 1 g in the revised USDA method decreased the level of sensitivity by an order of magnitude, as expected.
Agid:
5850142