PubAg

Main content area

Immune analysis of cry1Ab-genetically modified potato by in-silico analysis and animal model

Author:
Rahnama, Hassan, Nikmard, Mahdi, Abolhasani, Mohsen, Osfoori, Rahim, Sanjarian, Forough, Habashi, Ali Akbar
Source:
Food science and biotechnology 2017 v.26 no.5 pp. 1437-1445
ISSN:
1226-7708
Subject:
Phthorimaea operculella, Solanum tuberosum, adverse effects, allergens, amino acid sequences, animal models, blood serum, food intake, genes, genetic markers, health status, immunity, immunoglobulin A, immunoglobulin G, immunoglobulin M, interferon-gamma, interleukin-6, potatoes, rats, toxins, transgenic plants, tubers
Abstract:
The safety of feeding transgenic potato (Solanum tuberosum), resistant to the potato tuber moth, to Wistar rats was examined from an immunological perspective. The genetically modified potato (GMP) was harbouring cry1Ab and nptII genes as target and selectable marker genes, respectively. In-silico analysis reconfirmed that Cry1Ab and NPTII protein sequences have no significant homology to known toxins or known allergens. The Wistar rats were fed a diet containing 20% GMP or its parental control, non-GMP (NGMP), for 90 days. The consumption of GMP food did not affect the growth rate, food intake, food efficiency, and general health status of the rats. There were no significant differences in the serum concentrations of immunoglobulin A (IgA), IgG, IgM, interleukin 6 (IL-6), and IFN-γ between GMP and NGMP-fed rats. Based on such data, it is concluded that the transgenic potato had no adverse effect on immunity functions of Wistar rats.
Agid:
5850359