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A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
- Li, Song, Cao, Xupeng, Wang, Yan, Zhu, Zhen, Zhang, Haowei, Xue, Song, Tian, Jing
- Applied biochemistry and biotechnology 2017 v.183 no.3 pp. 923-930
- Chlamydomonas reinhardtii, biofuels, microalgae, models, proteomics
- With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.