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Evaluation of Chitosan-Based Films Containing Gelatin, Chondroitin 4-Sulfate and ZnO for Wound Healing

Cahú, Thiago B., Silva, Rodrigo A., Silva, Raquel P. F., Silva, Milena M., Arruda, Isabel R. S., Silva, Janilson F., Costa, Romero M. P. B., Santos, Suzan D., Nader, Helena B., Bezerra, Ranilson S.
Applied biochemistry and biotechnology 2017 v.183 no.3 pp. 765-777
biopolymers, cell growth, chitin, chitosan, erythrocytes, fibroblasts, gelatin, granulation tissue, hemolysis, histology, keratinocytes, molecular weight, processing waste, sugars, tissue repair, toxicity, zinc oxide
In this work, chitosan-based films containing gelatin and chondroitin-4-sulfate (C4S) with and without ZnO particles were produced and tested in vitro to investigate their potential wound healing properties. Chitosans were produced from shrimp-head processing waste by alkaline deacetylation of chitin to obtain chitosans differing in molecular weight and degree of deacetylation (80 ± 0.5%). The film-forming solutions (chitosan, C4S and gelatin) and ZnO suspension showed no toxicity towards fibroblasts or keratinocytes. Chitosan was able to agglutinate red blood cells, and film-forming solutions induced no hemolysis. Film components were released into solution when incubated in PBS as demonstrated by protein and sugar determination. These data suggest that a stable, chitosan-based film with low toxicity and an ability to release components would be able to establish a biocompatible microenvironment for cell growth. Chitosan-based films significantly increased the percentage of wound healing (wound contraction from 65 to 86%) in skin with full-thickness excision when compared with control (51%), after 6 days. Moreover, histological analysis showed increased granulation tissue in chitosan and chitosan/gelatin/C4S/ZnO films. Chitosan-based biopolymer composites could be used for improved biomedical applications such as wound dressings, giving them enhanced properties.