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Stx1 prophage excision in Escherichia coli strain PA20 confers strong curli and biofilm formation by restoring native m1ra

Author:
Uhlich, Gaylen A., Chen, Chin-Yi, Cottrell, Bryan J., Hofmann, Christopher S., Yan, Xianghe, Nguyen, Ly
Source:
FEMS Microbiology Letters 2016 v.363 no.13 pp. 1-8
ISSN:
0378-1097
Subject:
Escherichia coli O157, biofilm, fimbriae, lysogeny, mutation, phenotype, regulator genes, transcriptomics
Abstract:
Prophage insertions in Escherichia coli O157:H7 mlrA contribute to the low expression of curli fimbriae and biofilm observed in many clinical isolates. Varying levels of CsgD-dependent curli/biofilm expression are restored to strains bearing prophage insertions in mlrA by mutation of regulatory genes affecting csgD . Our previous study identified strong biofilm- and curli-producing variants in O157:H7 cultures that had lost the mlrA -imbedded prophage characteristic of the parent population, suggesting prophage excision as a mechanism for restoring biofilm properties. In this study, we compared genomic, transcriptomic and phenotypic properties of parent strain PA20 (stx1), stx2) and its prophage-cured variant, 20R2R (stx 2), and confirmed the mechanism underlying the differences in biofilm formation.
Agid:
5852150
Handle:
10113/5852150