U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Stx1 prophage excision in Escherichia coli strain PA20 confers strong curli and biofilm formation by restoring native m1ra

Author:
Gaylen A. Uhlich, Chin-Yi Chen, Bryan J. Cottrell, Christopher S. Hofmann, Xianghe Yan, Ly Nguyen
Source:
FEMS Microbiology Letters 2016 v.363 no.13 pp. 1-8
ISSN:
0378-1097
Subject:
Escherichia coli O157, biofilm, fimbriae, lysogeny, mutation, phenotype, regulator genes, transcriptomics
Abstract:
Prophage insertions in Escherichia coli O157:H7 mlrA contribute to the low expression of curli fimbriae and biofilm observed in many clinical isolates. Varying levels of CsgD-dependent curli/biofilm expression are restored to strains bearing prophage insertions in mlrA by mutation of regulatory genes affecting csgD . Our previous study identified strong biofilm- and curli-producing variants in O157:H7 cultures that had lost the mlrA -imbedded prophage characteristic of the parent population, suggesting prophage excision as a mechanism for restoring biofilm properties. In this study, we compared genomic, transcriptomic and phenotypic properties of parent strain PA20 (stx1), stx2) and its prophage-cured variant, 20R2R (stx 2), and confirmed the mechanism underlying the differences in biofilm formation.
Agid:
5852150
Handle:
10113/5852150