U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Maternal low protein diet leads to placental angiogenic compensation via dysregulated M1/M2 macrophages and TNFa expression in Sprague-Dawley rats

Author:
Emilie Vomhof-DeKrey, Diane Darland, Othman Ghribi, Amy Bundy, James Roemmich, Kate Claycombe
Source:
Journal of Reproductive Immunology 2016 v.118 no. pp. 9-17
ISSN:
0165-0378
Subject:
adipose tissue, adults, angiogenesis, animal feeding, animal models, cell adhesion molecules, compensatory growth, dams (mothers), endothelial cells, fetal development, fetus, insulin resistance, low birth weight, low protein diet, macrophages, malnutrition, maternal nutrition, obesity, placenta, pregnancy, protein content, rats, secretion, tumor necrosis factor-alpha, vascular endothelial growth factor receptor-1
Abstract:
A maternal low-protein (LP) diet in Sprague-Dawley rats results in low birth weight, rapid adipose tissue catch-up growth, adult obesity, and insulin resistance. The placenta functions to fulfill the fetus' nutrient demands. Adequate angiogenic factor concentrations help to ensure normal growth and vasculature development of the placenta and, in turn, optimum maternal-to-fetal nutrient delivery. Maternal malnutrition creates a proinflammatory environment that leads to inhibition of placental tissue growth. Therefore, we hypothesized that a maternal LP diet will lead to abnormal angiogenesis via dysregulation of immune cells resulting in increased secretion of proinflammatory cytokines and reduced angiogenic factor expression. Sprague-Dawley dams were fed 8% LP or 20% normal protein diets for 3 weeks prior to breeding and throughout pregnancy. Placenta from dams fed a LP diet weighed less; had increased M2 macrophages producing TNFα, decreased M1 macrophages and iNKT cells; greater angiogenic factor (FGF2, VEGFR-1, IGF2) expression and protein content, and greater CD31/PECAM (platelet endothelial cell adhesion molecule) expression. Prenatal protein restriction may induce the placenta to upregulate compensatory mechanisms of angiogenesis in order to meet the nutrient demands of the fetus.
Agid:
5852157
Handle:
10113/5852157