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DcSto – carrot Stowaway-like elements are abundant, diverse, and polymorphic
- Macko-Podgorni, Alicja, Nowicka, Anna, Grzebelus, Ewa, Simon, Philipp W., Grzebelus, Dariusz
- Genetica 2013 v.141 no.4-6 pp. 255
- Daucus carota, carrots, chromosomes, gene expression regulation, genes, genetic polymorphism, insertional mutagenesis, promoter regions, terminal repeat sequences, transposons
- We investigated nine families of Stowaway-like MITEs in the carrot genome, named DcSto1 to DcSto9. All of them were AT-rich and shared a highly conserved 6 bp-long TIR typical for Stowaways. The copy number of DcSto1 elements was estimated as ca. 5,000 per diploid genome. We observed preference for clustered insertions of DcSto and other MITEs. Distribution of DcSto1 hybridization signals revealed presence of DcSto1 clusters within euchromatic regions along all chromosomes. An arrangement of eight regions encompassing DcSto insertion sites, studied in detail, was highly variable among plants representing different populations of Daucus carota. All of these insertions were polymorphic which most likely suggests a very recent mobilization of those elements. Insertions of DcSto near carrot genes and presence of putative promoters, regulatory motifs, and polyA signals within their sequences might suggest a possible involvement of DcSto in the regulation of gene expression.