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Optimization, antioxidant properties and GC–MS analysis of Periploca angustifolia polysaccharides and chelation therapy on cadmium-induced toxicity in human HepG2 cells line and rat liver

Athmouni, Khaled, Belhaj, Dalel, El Feki, Abdelfattah, Ayadi, Habib
International journal of biological macromolecules 2018 v.108 pp. 853-862
bilirubin, polysaccharides, oral administration, liver function, cell viability, males, cadmium, gas chromatography-mass spectrometry, human cell lines, humans, body weight, cytotoxicity, superoxide dismutase, response surface methodology, rats, antioxidant activity, histopathology, antioxidants, liver, catalase, solvents, cadmium chloride, chelation
The extraction of Periploca polysaccharides (PAPS) was optimized using the response surface methodology. The influence of solvent, liquid-solid ratio and extraction time on polysaccharide yield was evaluated using a full factorial design (23). Also, PAPS extract did not induce a cytotoxic effect on HepG2 cells within the range of tested concentrations (0–250μgmL−1). Herein, the pre-treatment with PAPS extract (100μgmL−1) reduced cell mortality. Furthermore, the in vivo antioxidant activity of PAPS extract was investigated in rats. The oral administration of 250mgkg−1 body weight of PAPS extract administered above a period of 10 weeks to cadmium chloride (CdCl2) induced toxicity in male Wistar rats, markedly decreased the content of MDA and protein damage in liver tissue, and enhanced liver function parameters (ALAT, ASAT and bilirubin), as well as the activities of hepatic antioxidant status (SOD, CAT, GPx and GSH). Finally, the examination of liver histopathology confirmed that PAPS ameliorate the alteration of liver tissue caused by exposition to cadmium.