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Induction of new metabolites from the endophytic fungus Bionectria sp. through bacterial co-culture

Kamdem, Ramsay S.T., Wang, Hao, Wafo, Pascal, Ebrahim, Weaam, Özkaya, Ferhat Can, Makhloufi, Gamall, Janiak, Christoph, Sureechatchaiyan, Parichat, Kassack, Matthias U., Lin, Wenhan, Liu, Zhen, Proksch, Peter
Fitoterapia 2017
Bacillus subtilis, Clonostachys, Streptomyces lividans, alkaloids, anthranilic acid, axenic culture, bacteria, coculture, crystal structure, cytotoxicity, endophytes, fungi, human cell lines, inhibitory concentration 50, medicinal plants, metabolites, multigene family, nuclear magnetic resonance spectroscopy, ovarian neoplasms, seeds, spectral analysis, tropical plants
A new alkaloid, 1,2-dihydrophenopyrrozin (1), along with five known compounds (2–6) was isolated from an axenic culture of the endophytic fungus, Bionectria sp., obtained from seeds of the tropical plant Raphia taedigera. Co-cultivation of this fungus either with Bacillus subtilis or with Streptomyces lividans resulted in the production of two new o-aminobenzoic acid derivatives, bionectriamines A and B (7 and 8) as well as of two additional known compounds (9 and 10). None of the latter compounds (7–10) were detected in axenic cultures of the fungus or of the bacteria indicating activation of silent biogenetic gene clusters through co-cultivation with bacteria. The structures of the new compounds were unambiguously determined based on detailed NMR and MS spectroscopic analysis and by comparison with the literature. The crystal structure of agathic acid (6) is reported here for the first time. Penicolinate A (4) exhibited potent cytotoxic activity against the human ovarian cancer cell line A2780 with an IC50 value of 4.1μM.