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Effect of dietary leucine on growth performance, hemolymph and hepatopancreas enzyme activities of swimming crab, Portunus trituberculatus

Huo, Y.‐W., Jin, M., Sun, P., Hou, Y.‐M., Li, Y., Qiu, H., Zhou, Q.‐C.
Aquaculture nutrition 2017 v.23 no.6 pp. 1341-1350
Portunus trituberculatus, alanine transaminase, antioxidants, aspartate transaminase, blood serum, cement, cholesterol, crabs, crude protein, dietary protein, enzyme activity, feed conversion, glucose, growth performance, hemolymph, hepatopancreas, juveniles, leucine, malondialdehyde, models, monophenol monooxygenase, protein efficiency ratio, rearing, superoxide dismutase, triacylglycerols, weight gain
An 8‐week feeding trial was conducted to determine the dietary leucine requirement for juvenile swimming crabs reared in cement pools. Six isonitrogenous and isolipidic practical diets (430 g/kg crude protein and 70 g/kg crude lipid) were formulated to contain graded leucine levels which ranged from 16.7 to 26.7 g/kg (dry weight). Each diet was randomly assigned to triplicate groups of 60 juvenile swimming crabs (initial average weight 3.75 ± 0.12 g) that were stocked in rectangle plastic baskets. The results of the present study indicated that dietary leucine levels significantly influenced weight gain (WG) and specific growth ratio (SGR) (p < .05), crab fed the diet containing 22.7 g/kg leucine had significantly higher WG and SGR than those fed the other diets. Feed efficiency and protein efficiency ratio were not significantly affected by the dietary leucine levels (p > .05). Total protein, cholesterol, triglyceride and glucose in serum were significantly affected by the dietary leucine levels. Aspartate aminotransferase (AST) and alanine aminotransferase activities in hemolymph, AST and superoxide dismutase activities in hepatopancreas were significantly affected by dietary leucine levels; moreover, crab fed the 16.7 g/kg leucine diet had higher malondialdehyde in hemolymph and hepatopancreas than those fed the other diets. Crab fed the diet containing 24.9 g/kg leucine had higher phenoloxidase activity in hemolymph than those fed the other diets. Based on two‐slope broken‐line model of SGR against dietary leucine levels, the optimal dietary leucine requirement for growth was estimated to be 22.1 g/kg of the dry diet (corresponding to 51.4 g/kg of dietary protein on a dry weight basis). In summary, findings of this study indicated that dietary leucine could improve growth performance and antioxidant status.