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Physiological responses of great sturgeon (Huso huso) to different concentrations of 2-phenoxyethanol as an anesthetic

Author:
Shaluei, Fardin, Hedayati, Aliakbar, Jahanbakhshi, Abdolreza, Baghfalaki, Maryam
Source:
Fish physiology and biochemistry 2012 v.38 no.6 pp. 1627-1634
ISSN:
0920-1742
Subject:
Huso huso, alanine transaminase, alkaline phosphatase, anesthesia, anesthetics, aspartate transaminase, blood glucose, cholesterol, cortisol, electrolytes, erythrocytes, hematocrit, hemoglobin, leukocytes, metabolites, physiological response, sturgeon, triacylglycerols, Alps region
Abstract:
The objective of the study was to evaluate the efficacy of 2-phenoxyethanol (2-PE) as an anesthetic in great sturgeon under two experiments. First, fish were exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL/L 2-PE, and time to induction (deep anesthesia) and recovery from anesthesia were measured. At concentration of 0.1 mL/L, 2-PE failed to induce deep anesthesia in fish, whereas at concentrations of 0.7 and 0.9 mL/L, all the fish were anaesthetized within 3 min of exposure. For assessing the impact of effective concentrations of 2-PE on physiological responses of great sturgeon, hematological indices, plasma metabolites, electrolytes, enzymes and cortisol levels were measured. The use of 2-PE induces a significant increase in RBC values at 0.3 mL/L concentration and a parallel increase in hemoglobin and hematocrit values. 2-PE anesthesia had no effect on WBC, MCV, MCH and MCHC levels when compared to control group. Serum glucose, cholesterol and cortisol levels were significantly high in 0.3 and 0.5 mL/L 2-PE. Moreover, AST levels were increased in fish exposed to the 0.3 mL/L 2-PE comparing with the control group (P < 0.05). There were no significant differences in serum levels of total protein, triglycerides, ALP, ALT, Cl⁻, Na⁺, K⁺, Ca²⁺ and Mg²⁺. In this study, alteration in hematological and serum biochemical indices was time-dependent. This study demonstrates that rapid induction of deep anesthesia with a relatively high concentration of 2-PE (0.7 and 0.9 mL/L) was associated with the lowest effects on the hematological and serum biochemical indices in great sturgeon and therefore would be recommended as eligible doses for hematological studies in this species.
Agid:
585828