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Determination of Fusaric Acid in Maize Using Molecularly Imprinted SPE Clean-Up

Author:
Michael Appell, Michael A. Jackson, Lijuan C. Wang, Che-Hin Ho, Anja Mueller
Source:
Journal of Separation Science 2014 v.37 pp. 281-286
ISSN:
1615-9306
Subject:
corn, fusaric acid, hydrogen, liquid chromatography, picolinic acid, polymers
Abstract:
A new liquid chromatography method to detect fusaric acid (FA)in maize is reported based on molecularly imprinted polymer SPE clean-up (MISPE) using mimic-templated molecularly-imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers with methacrylic acid or 2-dimethylaminoethyl methacrylate functional monomers exhibited imprinting effects in SPE analysis. Fusaric acid levels were determined using reversed phase ion-pairing chromatography and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect fusaric acid in maize using MISPE analysis within the range of 1-100 µg/g with recoveries between 83.9-92.1%.
Agid:
58584
Handle:
10113/58584