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Erythorbyl laurate as a potential food additive with multi-functionalities: Antibacterial activity and mode of action

Park, Kyung-Min, Jo, Su-Kyung, Yu, Hyunjong, Park, Jun-Young, Choi, Seung Jun, Lee, Chang Joo, Chang, Pahn-Shick
Food control 2018 v.86 pp. 138-145
antibacterial properties, cell membranes, erythromycin, food additives, food pathogens, gentian violet, growth curves, kanamycin, mechanism of action, membrane permeability, minimum inhibitory concentration, nisin, screening, specific growth rate, synergism
The antibacterial activity and mechanism of erythorbyl laurate were investigated to provide information on practical applications as a multi-functional food additive. Antibacterial susceptibility screening demonstrated that erythorbyl laurate exerted both bacteriostatic and bactericidal effects on gram-positive foodborne pathogens (minimum inhibitory concentration: 0.48–0.88 mM, minimum bactericidal concentration: 0.65–1.00 mM). The growth curves of gram-positive strains showed increases in the λ (lag phase) accompanied by decreases in the μmax (maximum specific growth rate) as the concentration of erythorbyl laurate increased. The results of the crystal violet uptake and LIVE/DEAD BacLight assays suggested that the antibacterial mechanism of erythorbyl laurate might depend on alterations in the permeability and integrity of cell membranes. Furthermore, erythorbyl laurate showed strong synergistic effects when combined with a variety of antibacterial agents including nisin, kanamycin, and erythromycin (FIC index < 0.28), which might result from increases in bacterial membrane permeability induced by erythorbyl laurate.