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Selenium treatment differentially affects sulfur metabolism in high and low glucosinolate producing cultivars of broccoli (Brassica oleracea L.)

Author:
McKenzie, Marian J., Chen, Ronan K.Y., Leung, Susanna, Joshi, Srishti, Rippon, Paula E., Joyce, Nigel I., McManus, Michael T.
Source:
Plant physiology and biochemistry 2017 v.121 pp. 176-186
ISSN:
0981-9428
Subject:
Brassica oleracea, broccoli, cultivars, cysteine synthase, fertilizer application, germplasm, glucosinolates, human health, metabolism, selenium, selenium fertilizers, sulfate adenylyltransferase, sulfur, thiols
Abstract:
The effect of selenium (Se) application on the sulfur (S)-rich glucosinolate (GSL)-containing plant, broccoli (Brassica oleracea L. var. italica) was examined with a view to producing germplasm with increased Se and GSL content for human health, and to understanding the influence of Se on the regulation of GSL production. Two cultivars differing in GSL content were compared. Increased Se application resulted in an increase in Se uptake in planta, but no significant change in total S or total GSL content in either cultivar. Also no significant change was observed in the activity of ATP sulfurylase (ATPS, EC 2.7.7.4) or O-acetylserine(thiol) lyase (OASTL, EC 2.5.1.47) with increased Se application. However, in the first investigation of APS kinase (APSK, EC 2.7.1.25) expression in response to Se fertilisation, an increase in transcript abundance of one variant of APS kinase 1 (BoAPSK1A) was observed in both cultivars, and an increase in BoAPSK2 transcript abundance was observed in the low GSL producing cultivar. A mechanism by which increased APSK transcription may provide a means of controlling the content of S-containing compounds, including GSLs, following Se uptake is proposed.
Agid:
5859288