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An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

Author:
Georgia E. Guild, Nicholas G. Paltridge, Meike S. Andersson, James C. R. Stangoulis
Source:
Plant and soil 2017 v.419 no.1-2 pp. 457-466
ISSN:
0032-079X
Subject:
X-ray fluorescence spectroscopy, biofortification, breeding programs, corn, corn flour, cowpeas, crops, energy-dispersive X-ray analysis, genotype, iron, mass spectrometry, rapid methods, screening, zinc
Abstract:
BACKGROUND AND AIMS: Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. METHODS: Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. RESULTS: Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg⁻¹ for both Fe and Zn. CONCLUSION: EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.
Agid:
5860149