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Multimycotoxin LC–MS/MS Analysis in Tea Beverages after Dispersive Liquid–Liquid Microextraction (DLLME)

Pallarés, Noelia, Font, Guillermina, Mañes, Jordi, Ferrer, Emilia
Journal of agricultural and food chemistry 2017 v.65 no.47 pp. 10282-10289
aflatoxin B2, aflatoxin G1, aflatoxin G2, black tea, detection limit, green tea, liquid chromatography, liquid-phase microextraction, mint, risk assessment, tandem mass spectrometry, tea (beverage)
The aim of the present study was to develop a multimycotoxin liquid chromatography tandem mass spectrometry (LC–MS/MS) method with a dispersive liquid–liquid microextraction procedure (DLLME) for the analysis of AFs, 3aDON, 15aDON, NIV, HT-2, T-2, ZEA, OTA, ENNs, and BEA in tea beverages and to evaluate their mycotoxin contents. The proposed method was characterized in terms of linearity, limits of detection (LODs), limits of quantification (LOQs), recoveries, repeatability (intraday precision), reproducibility (interday precision), and matrix effects to check suitability. The results show LODs in the range of 0.05–10 μg/L, LOQs in the range of 0.2–33 μg/L, and recoveries in the range of 65–127% (RSD < 20%). The method developed in this study was applied to 44 commercial samples of black tea, red tea, green tea, and green mint tea. The results show that, of the analyzed mycotoxins, AFB2, AFG2, 15aDON, AFG1, and ENB were detected in the samples. AFB2 (14.4–32.2 μg/L) and 15aDON (60.5–61 μg/L) presented the highest levels. Green mint tea contained the highest concentration of mycotoxins. The risk assessment study shows that the population is not much exposed to mycotoxins through the consumption of tea beverages.