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Exploration of Potentially Bioactive Peptides Generated from the Enzymatic Hydrolysis of Hempseed Proteins

Author:
Aiello, Gilda, Lammi, Carmen, Boschin, Giovanna, Zanoni, Chiara, Arnoldi, Anna
Source:
Journal of agricultural and food chemistry 2017 v.65 no.47 pp. 10174-10184
ISSN:
1520-5118
Subject:
active sites, antioxidant activity, dipeptidyl-peptidase IV, enzymatic hydrolysis, enzyme activity, functional foods, glucose, hemp, hydrolysates, pancreatin, pepsin, peptides, peptidyl-dipeptidase A, protein concentrates, protein sources, trypsin
Abstract:
The seed of industrial hemp is an underexploited protein source. In view of a possible use in functional foods, a hempseed protein concentrate was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these enzymes. A detailed peptidomic analysis using data-dependent acquisition showed that the numbers of peptides identified ranged from 90 belonging to 33 parent proteins in the peptic hydrolysate to 9 belonging to 6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme A reductase activity when tested on the catalytic domain of the enzyme. Using the open access tools PeptideRanker and BIOPEP, a list of potentially bioactive peptides was generated: the alleged activities included the antioxidant property, the glucose uptake stimulating activity, the inhibition of dipeptidyl peptidase-IV and angiotensin-converting enzyme I.
Agid:
5864291