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Mutations of Residues in Pocket P1 of a Cyclodipeptide Synthase Strongly Increase Product Formation

Author:
Brockmeyer, Kirsten, Li, Shu-Ming
Source:
Journal of natural products 2017 v.80 no.11 pp. 2917-2922
ISSN:
1520-6025
Subject:
Escherichia coli, Nocardiopsis prasina, amino acids, genes, mutants, site-directed mutagenesis
Abstract:
Expression of a cyclodipeptide synthase gene from Nocardiopsis prasina (CDPS-Np) in Escherichia coli resulted in the formation of cyclo-(l-Tyr-l-Tyr) (1) as the minor and cyclo-(l-Tyr-l-Phe) (2) as the major products. Site-directed mutagenesis revealed a strong influence on product accumulation of the amino acid residues in pocket P1. An 8-fold increase in product formation for 1 and 10-fold for 2 were detected in the double mutant T82V_Y196F compared with the wild type.
Agid:
5864321