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Neutralising ability of Terminalia fagifolia extract (Combretaceae) against the in vitro neuromuscular effects of Bothrops jararacussu venom
- Tribuiani, Natália, Tavares, Marylu Oliveira, Santana, Monique Neves, Fontana Oliveira, Isadora Caruso, Amaral Filho, Jorge do, Silva, Magali Glauzer, dos Santos, Marcio Galdino, Cogo, José Carlos, Floriano, Rafael Stuani, Cogo-Müller, Karina, Oshima-Franco, Yoko
- Natural product research 2017 v.31 no.23 pp. 2783-2787
- Bothrops, Terminalia, chicks, creatine kinase, histology, mice, neutralization, paralysis, receptors, saponins, toxicity, venoms
- The ability of Terminalia fagifolia hydroalcoholic extract (Tf -HE) to neutralise the paralysis and myotoxicity induced by Bothrops jararacussu venom was assayed using mouse phrenic nerve-diaphragm (PND) preparation and two varieties of chick biventer cervicis (BC) preparations. Tf -HE 100 μg/mL and 500 μg/mL were tested against 40 and 200 μg of venom/mL in PND and BC preparations, respectively, using pre- and post-venom incubation treatments. The effects of Tf -HE against the myotoxicity caused by venom were evaluated via histological analysis (PND) and creatine kinase (CK) release (BC). Tf -HE was able to reverse the venom paralysis in both preparation types. The contractures to exogenous ACh in BC preparations showed that Tf -HE may act on extrinsic, preserving those intrinsic postsynaptic receptors. There was a positive correlation between CK and morphological changes. The high non-hemolytic saponin content can explain the Tf -HE efficacy against the toxic effects of B. jararacussu venom in vertebrate neuromuscular preparations.