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Neutralising ability of Terminalia fagifolia extract (Combretaceae) against the in vitro neuromuscular effects of Bothrops jararacussu venom

Tribuiani, Natália, Tavares, Marylu Oliveira, Santana, Monique Neves, Fontana Oliveira, Isadora Caruso, Amaral Filho, Jorge do, Silva, Magali Glauzer, dos Santos, Marcio Galdino, Cogo, José Carlos, Floriano, Rafael Stuani, Cogo-Müller, Karina, Oshima-Franco, Yoko
Natural product research 2017 v.31 no.23 pp. 2783-2787
Bothrops, Terminalia, chicks, creatine kinase, histology, mice, neutralization, paralysis, receptors, saponins, toxicity, venoms
The ability of Terminalia fagifolia hydroalcoholic extract (Tf -HE) to neutralise the paralysis and myotoxicity induced by Bothrops jararacussu venom was assayed using mouse phrenic nerve-diaphragm (PND) preparation and two varieties of chick biventer cervicis (BC) preparations. Tf -HE 100 μg/mL and 500 μg/mL were tested against 40 and 200 μg of venom/mL in PND and BC preparations, respectively, using pre- and post-venom incubation treatments. The effects of Tf -HE against the myotoxicity caused by venom were evaluated via histological analysis (PND) and creatine kinase (CK) release (BC). Tf -HE was able to reverse the venom paralysis in both preparation types. The contractures to exogenous ACh in BC preparations showed that Tf -HE may act on extrinsic, preserving those intrinsic postsynaptic receptors. There was a positive correlation between CK and morphological changes. The high non-hemolytic saponin content can explain the Tf -HE efficacy against the toxic effects of B. jararacussu venom in vertebrate neuromuscular preparations.