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New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins

Orio, Lara P., Boschin, Giovanna, Recca, Teresa, Morelli, Carlo F., Ragona, Laura, Francescato, Pierangelo, Arnoldi, Anna, Speranza, Giovanna
Journal of agricultural and food chemistry 2017 v.65 no.48 pp. 10482-10488
Cannabis sativa, acid deposition, hemp, hydrochloric acid, hydrolysates, hydrolysis, inhibitory concentration 50, mass spectrometry, nuclear magnetic resonance spectroscopy, peptides, peptidyl-dipeptidase A, protein isolates, reversed-phase high performance liquid chromatography, solubilization
A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC₅₀ values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.