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New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins
- Orio, Lara P., Boschin, Giovanna, Recca, Teresa, Morelli, Carlo F., Ragona, Laura, Francescato, Pierangelo, Arnoldi, Anna, Speranza, Giovanna
- Journal of agricultural and food chemistry 2017 v.65 no.48 pp. 10482-10488
- Cannabis sativa, acid deposition, hemp, hydrochloric acid, hydrolysates, hydrolysis, inhibitory concentration 50, mass spectrometry, nuclear magnetic resonance spectroscopy, peptides, peptidyl-dipeptidase A, protein isolates, reversed-phase high performance liquid chromatography, solubilization
- A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC₅₀ values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.