Main content area

Development of polymorphic microsatellite markers of the common toad, Duttaphrynus melanostictus useful for genetic studies

Janani, S. Jegath, Sharma, Richa, Vasudevan, Karthikeyan, Dutta, Sushil K., Aggarwal, Ramesh K.
Conservation genetics resources 2013 v.5 no.2 pp. 537-540
alleles, genomic libraries, geographical distribution, habitats, loci, microsatellite repeats, toads, Asia
Asian common toad, Duttaphrynus melanostictus, is a widespread habitat generalist species of Indian-subcontinent and South-East Asia. Here we report, 14 new microsatellite markers (SSRs) for this toad, developed from a SSR-enriched genomic library, and validated using 44 unrelated samples of Asian toad and ~20 samples belonging to four congeners. Nine SSRs were highly polymorphic; amplified 6–22 alleles/marker with H ₒ and PIC ranging from 0.477 to 0.955 and 0.518 to 0.918, respectively, while one was found to be monomorphic. Of the remaining four SSRs, two (DMccmb02, DMccmb17) amplified only two apparently fixed alleles in all samples, while the other two (DMccmb22, DMccmb29) amplified 1–4alleles/sample; these four SSRs probably represent duplicated loci. Interestingly, all the 14 SSRs showed cross-species transferability to varying extent, with four SSRs showing 100 % transferability. It is hoped that these SSRs will greatly facilitate population genetic studies on the Asian toad species across their geographic distribution.