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α-Methyl artoflavanocoumarin from Juniperus chinensis exerts anti-diabetic effects by inhibiting PTP1B and activating the PI3K/Akt signaling pathway in insulin-resistant HepG2 cells

Author:
Jung, Hee Jin, Seong, Su Hui, Ali, Md Yousof, Min, Byung-Sun, Jung, Hyun Ah, Choi, Jae Sue
Source:
Archives of pharmacal research 2017 v.40 no.12 pp. 1403-1413
ISSN:
0253-6269
Subject:
Juniperus chinensis, diabetes mellitus, flavonoids, glucose, glycemic effect, human cell lines, inhibitory concentration 50, insulin, insulin receptor substrate proteins, insulin resistance, mitogen-activated protein kinase, molecular models, phosphatidylinositol 3-kinase, phosphorylation, protein-tyrosine-phosphatase, signal transduction
Abstract:
Diabetes mellitus is one of the greatest global health issues and much research effort continues to be directed toward identifying novel therapeutic agents. Insulin resistance is a challenging integrally related topic and molecules capable of overcoming it are of considerable therapeutic interest in the context of type 2 diabetes mellitus (T2DM). Protein tyrosine phosphatase 1B (PTP1B) negatively regulates insulin signaling transduction and is regarded a novel therapeutic target in T2DM. Here, we investigated the inhibitory effect of α-methyl artoflavanocoumarin (MAFC), a natural flavanocoumarin isolated from Juniperus chinensis, on PTP1B in insulin-resistant HepG2 cells. MAFC was found to potently inhibit PTP1B with an IC₅₀ of 25.27 ± 0.14 µM, and a kinetics study revealed MAFC is a mixed type PTP1B inhibitor with a K ᵢ value of 13.84 µM. Molecular docking simulations demonstrated MAFC can bind to catalytic and allosteric sites of PTP1B. Furthermore, MAFC significantly increased glucose uptake and decreased the expression of PTP1B in insulin-resistant HepG2 cells, down-regulated the phosphorylation of insulin receptor substrate (IRS)-1 (Ser307), and dose-dependently enhanced the protein levels of IRS-1, phosphorylated phosphoinositide 3-kinase (PI3K), Akt, and ERK1. These results suggest that MAFC from J. chinensis has therapeutic potential in T2DM by inhibiting PTP1B and activating insulin signaling pathways.
Agid:
5868495