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Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite
- He, Li-Sheng, Zhang, Gen, Wang, Yong, Yan, Guo-Yong, Qian, Pei-Yuan
- Biochemical and biophysical research communications 2018 v.495 no.1 pp. 969-975
- Balanus amphitrite, Pollicipes, adults, cement, cement glands, dithiothreitol, fluorescence microscopy, fouling, proteins, urea, vacuoles
- Barnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized from Amphibalanus amphitrite in this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles including Megabalanus rosa (Mr-cp100k), Tetraclita japonica formosana (Tj-cp100k) and Pollicipes pollicipes (Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid β secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that in Megabalanus rosa, implying diverse possible roles of cp100k in cementing.