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An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress

Xu, Yang, Hu, Dongwen, Li, Yuting, Sun, Chongde, Chen, Wei
Journal of Chromatography B 2017
anthocyanins, antioxidant activity, countercurrent chromatography, freeze drying, ingredients, mitochondria, oxidative stress, porous media, protective effect, strawberries
Accumulating evidence indicates that consumption of berries may exert beneficial effects against oxidative stress mediated diseases. Pelargonidin-3-O-glucoside (Pg3G), a bioactive ingredient in strawberry, has been reported to possess a potent antioxidant capacity. This study was therefore designed to develop an effective method to prepare pure Pg3G from strawberry and investigate its protective effect against H2O2-induced oxidative stress. According to our results, Pg3G occupied 85.55% of total anthocyanin content in strawberry. 240mg of Pg3G with the purity of 97.26% was finally isolated from 320g of strawberry lyophilized powder (SLP) by combination of AB-8 macroporous resin and high-speed counter-current chromatography (HSCCC) technologies. Further study unveiled that Pg3G significantly inhibited H2O2-induced ROS generation, GSH depletion and mitochondrial dysfunction, thereby ameliorating H2O2-induced oxidative stress. Overall, this study suggests that pelargonidin-3-O-glucoside can be used as a natural bioactive agent to prevent cellular oxidative stress.