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Gastrointestinal microbial diversity and short-chain fatty acid production in pigs fed different fibrous diets with or without cell wall-degrading enzyme supplementation

Author:
Zhang, Y.J., Liu, Q., Zhang, W.M., Zhang, Z.J., Wang, W.L., Zhuang, S.
Source:
Livestock science 2018 v.207 pp. 105-116
ISSN:
1871-1413
Subject:
Lachnospiraceae, Lactobacillus, Methanobrevibacter, Peptostreptococcaceae, Ruminococcaceae, Streptococcaceae, Treponema, Veillonella, acetates, animal production, bacteria, diet, digesta, endo-1,4-beta-glucanase, feces, feeds, ileum, phytases, propionic acid, short chain fatty acids, soybeans, swine, swine feeding, wheat, xylanases
Abstract:
The microbial diversity and short-chain fatty acid (SCFA) production in pigs fed different fibrous diets with or without cell wall-degrading enzyme (phytase, cellulase and xylanase) supplementation were compared using the Illumina MiSeq sequencing technique. Eight growing castrated pigs fitted with a T-shaped cannula at the terminal ileum were randomly assigned to four diets, i.e., wheat bran-based diet (WB), enzyme-supplemented WB, soybean hull-based diet (SH), and enzyme-supplemented SH, according to a 4 × 4 Latin square design. The ileal digesta and feces were collected after 15 days of adaptation in each period. The WB diet increased (P < 0.05) the abundances of Lactobacillus, Veillonella and Ruminococcaceae compared to SH diet, whereas the SH diet led to a higher (P < 0.05) abundances of Streptococcaceae, Peptostreptococcaceae, Lachnospiraceae, Treponema and Methanobrevibacter than that of WB diet. Adding exogenous cell wall-degrading enzyme selectively altered the relative abundance of bacteria in both dietary groups. The SH promoted (P < 0.01) the concentrations of acetate, propionate and total SCFA in the ileal digesta and feces, and enzyme supplementation led to similar results for SCFA production (P < 0.01) but not for acetate in the feces compared to that of WB. These results may provide valuable information on how to utilize fibrous feedstuffs efficiently in animal production.
Agid:
5871031